There’s an article about Cellular Highways in Genetic Engineering and Biotechnology News this week. See here:
Come and meet us at flowcytometryUK in Edinburgh – we’re giving a talk on Wednesday 18th July 2018. As well as an overview of the technology, we will be showing off our latest results in the collaboration with GSK – demonstrating excellent results separating human PBMCs.
“Cell sorting with an inertial vortex: a new microfluidic technology for high-throughput research and clinical applications” 14:40, Wednesday 18th July 2018.
We gave our talk to a packed conference session at CYTO 2018 in Prague, also sharing the session with Dino DiCarlo, the father of inertial microfluidics. You can see our presentation here:
- TTP will be looking for cell therapy partners and presenting a Cellular Highways poster at ISCT 2018 entitled “A faster GMP cell sorter enabled by a microfluidic inertial vortex.”
Abstract: A new technology for a high-throughput GMP sorter
Many new concepts in cell therapy are hard to manufacture because they require a purification step that is challenging to achieve with any current technology. For example, several T cell therapies must process more than a billion cells at high purity, based on a combination of several molecular markers, in aseptic conditions. For various reasons, these requirements are hard to satisfy with commercial MACS and FACS or related instruments.
To solve this problem, we have invented a new type of fluorescence-based microfluidic cell sorter, to achieve high-throughput therapeutic cell sorting in GMP conditions. The core technology is based on the creation of an inertial vortex in a microfluidic channel by a thermal vapor bubble actuator, and achieves cell processing rates of up to 50 kHz, peak sort rates of 12 kHz in a lab-on-chip device of 250 microns width.
We demonstrate the physical operation of the sorter and experimental results on cells, showing high-fidelity sorting and high viability. We also present our ongoing work to multiplex this sorter technology and create a GMP sorter instrument, to process batches of greater than 109 cells at high purity and yield, in an automated aseptic consumable, and apply the sorter to new cell therapies.
- TTP will be presenting Cellular Highways at CYTO 2018 in Parallel Session 12: Cell Sorting and also participating in the Therapeutic Cell Sorting workshop and the CYTO Innovation session.
Abstract: Cell sorting by inertial microfluidics
We describe the invention of a new type of fluorescence-activated microfluidic cell sorter, enabled by an inertial vortex. Like devices created by several previous research groups, ours employs an electrical microheater to create fluid motion via the microscopic explosion of a thermal vapour bubble. However, unlike the previous devices, the fluid motion does not deflect a cell directly, but creates an inertial vortex, which flows downstream with the cell, gradually pushing it into the sort channel.
We have employed standard MEMS and lab-on-chip fabrication processes to create our sorter chip, and built simple instrumentation to test it: single-laser fluorescence and scattering optics for particle detection, real-time data processing on an FPGA, strobe imaging to measure individual sort events, and overall verification of sorting performance with fluorescence beads and fluorescently-labelled cells. We have also evaluated the sorter’s effect on viability of human cells via live/dead dyes and subculture measurements.
The current design of the inertial vortex sorter, which has been tested with particles and cells of diameter 6 – 15 µm, achieves a particle deflection window of 20 µs and cell processing rates of up to 50’000/s for individual cell addressability. We have achieved peak deflection rates of 5 kHz in the current device, although we believe that 12 kHz could be achieved with optimisation. The sorter is a very compact device with a lateral dimension of 250 µm including its input manifold, actuator and all channels: as far as we know it is the smallest microfluidic cell sorter yet built. This small size is an advantage for multiplexing on chip while maintaining efficient use of fluorescence optics.
In this presentation, the basic functionality and performance of a new technology in microfluidic cell sorting is demonstrated. The inertial vortex sorter has a number of interesting features, including the use of cheaper MEMS construction techniques and materials, the use of a much smaller vapour bubble, the absence of side-channels, and the compact size for multiplexing or lab-on-chip integration.